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dnmt 1 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc dnmt 1 antibody
    Dnmt 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt-1+antibody/anti+dnmt1/pm39304654-83-1-4
    Average 90 stars, based on 1 article reviews
    dnmt 1 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Gestational exposure to the AhR agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin induces BRCA-1 promoter hypermethylation and reduces BRCA-1 expression in mammary tissue of rat offspring: preventive effects of resveratrol.
    Article Snippet: Studieswithmurinemodels suggest thatmaternal exposure to aromatic hydrocarbon receptor (AhR) agonistsmay impair mammary gland differentiation and increase the susceptibility to mammary carcinogenesis in offspring.. However, the molecular mechanisms responsible for these perturbations remain largely unknown.. Previously, we reported that the AhR agonists 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced CpG methylation of the breast cancer-1 (BRCA-1) gene and reduced BRCA-1 expression in breast cancer cell lines.

    Article Title: Neuroprotective Effect of Polyherbal Recipe Containing Ginger, Chinese Date, and Wood Ear Mushroom against Ischemic Stroke with Metabolic Syndrome Condition via Epigenetic Modification of Inflammation and Oxidative Stress
    Article Snippet: At the end of an incubation period, they were incubated with one of the following primary antibodies: IL-6 (dilution 1 : 1000), TNF- α (dilution 1 : 1000), DNMT-1 (dilution 1 : 1000), HDAC-3 (dilution 1 : 1000), and β -actin (dilution 1 : 2000) (Cell Signaling Technology, USA) for 2 hours at 25°C.



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    Cell Signaling Technology Inc dnmt 1 antibody
    Dnmt 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dna methyltransferase dnmt  (Novus Biologicals)
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    Androgen receptor and paxillin interaction requires intact granulosa cell focal adhesions. ( A ) Proximity ligation assay (PLA) in primary mouse GCs treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and probed with antibodies against AR and PXN or AR and <t>DNMT</t> as negative control. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Right: red fluorescence was quantified in five randomly selected cells in each sample using Image J and analyzed with Mann–Whitney tests. Magnification: ×40. ** P < 0.01. ( B ) Representative western blot and band densitometry of primary mouse GCs (n = 3–6 mice per group) treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and analyzed by Mann–Whitney tests. * P < 0.05. ( C ) PLA assay in KGN cells edited with CRISPR to delete paxillin (PXN KO) or non-targeting control (NT CTRL) and treated with vehicle (VEH), 25 nM dihydrotestosterone (DHT) or 5 μM PF-573228 (PF). All samples were probed with PXN and AR antibodies. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Scale bar: 10 μm. AR: androgen receptor, PXN: paxillin, DNMT: <t>DNA</t> <t>methyltransferase</t> 1, GC: granulosa cells, VEH: vehicle, PF: PF-573228, FAK: focal adhesion kinase, CRISPR: clustered regularly interspaced short palindromic repeats, PXN KO: paxillin knockout, NT CTRL: non-targeting control. Uncropped blots are shown in .
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    Androgen receptor and paxillin interaction requires intact granulosa cell focal adhesions. ( A ) Proximity ligation assay (PLA) in primary mouse GCs treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and probed with antibodies against AR and PXN or AR and <t>DNMT</t> as negative control. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Right: red fluorescence was quantified in five randomly selected cells in each sample using Image J and analyzed with Mann–Whitney tests. Magnification: ×40. ** P < 0.01. ( B ) Representative western blot and band densitometry of primary mouse GCs (n = 3–6 mice per group) treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and analyzed by Mann–Whitney tests. * P < 0.05. ( C ) PLA assay in KGN cells edited with CRISPR to delete paxillin (PXN KO) or non-targeting control (NT CTRL) and treated with vehicle (VEH), 25 nM dihydrotestosterone (DHT) or 5 μM PF-573228 (PF). All samples were probed with PXN and AR antibodies. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Scale bar: 10 μm. AR: androgen receptor, PXN: paxillin, DNMT: <t>DNA</t> <t>methyltransferase</t> 1, GC: granulosa cells, VEH: vehicle, PF: PF-573228, FAK: focal adhesion kinase, CRISPR: clustered regularly interspaced short palindromic repeats, PXN KO: paxillin knockout, NT CTRL: non-targeting control. Uncropped blots are shown in .
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    Androgen receptor and paxillin interaction requires intact granulosa cell focal adhesions. ( A ) Proximity ligation assay (PLA) in primary mouse GCs treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and probed with antibodies against AR and PXN or AR and <t>DNMT</t> as negative control. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Right: red fluorescence was quantified in five randomly selected cells in each sample using Image J and analyzed with Mann–Whitney tests. Magnification: ×40. ** P < 0.01. ( B ) Representative western blot and band densitometry of primary mouse GCs (n = 3–6 mice per group) treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and analyzed by Mann–Whitney tests. * P < 0.05. ( C ) PLA assay in KGN cells edited with CRISPR to delete paxillin (PXN KO) or non-targeting control (NT CTRL) and treated with vehicle (VEH), 25 nM dihydrotestosterone (DHT) or 5 μM PF-573228 (PF). All samples were probed with PXN and AR antibodies. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Scale bar: 10 μm. AR: androgen receptor, PXN: paxillin, DNMT: <t>DNA</t> <t>methyltransferase</t> 1, GC: granulosa cells, VEH: vehicle, PF: PF-573228, FAK: focal adhesion kinase, CRISPR: clustered regularly interspaced short palindromic repeats, PXN KO: paxillin knockout, NT CTRL: non-targeting control. Uncropped blots are shown in .
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    Androgen receptor and paxillin interaction requires intact granulosa cell focal adhesions. ( A ) Proximity ligation assay (PLA) in primary mouse GCs treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and probed with antibodies against AR and PXN or AR and <t>DNMT</t> as negative control. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Right: red fluorescence was quantified in five randomly selected cells in each sample using Image J and analyzed with Mann–Whitney tests. Magnification: ×40. ** P < 0.01. ( B ) Representative western blot and band densitometry of primary mouse GCs (n = 3–6 mice per group) treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and analyzed by Mann–Whitney tests. * P < 0.05. ( C ) PLA assay in KGN cells edited with CRISPR to delete paxillin (PXN KO) or non-targeting control (NT CTRL) and treated with vehicle (VEH), 25 nM dihydrotestosterone (DHT) or 5 μM PF-573228 (PF). All samples were probed with PXN and AR antibodies. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Scale bar: 10 μm. AR: androgen receptor, PXN: paxillin, DNMT: <t>DNA</t> <t>methyltransferase</t> 1, GC: granulosa cells, VEH: vehicle, PF: PF-573228, FAK: focal adhesion kinase, CRISPR: clustered regularly interspaced short palindromic repeats, PXN KO: paxillin knockout, NT CTRL: non-targeting control. Uncropped blots are shown in .
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    Androgen receptor and paxillin interaction requires intact granulosa cell focal adhesions. ( A ) Proximity ligation assay (PLA) in primary mouse GCs treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and probed with antibodies against AR and PXN or AR and <t>DNMT</t> as negative control. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Right: red fluorescence was quantified in five randomly selected cells in each sample using Image J and analyzed with Mann–Whitney tests. Magnification: ×40. ** P < 0.01. ( B ) Representative western blot and band densitometry of primary mouse GCs (n = 3–6 mice per group) treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and analyzed by Mann–Whitney tests. * P < 0.05. ( C ) PLA assay in KGN cells edited with CRISPR to delete paxillin (PXN KO) or non-targeting control (NT CTRL) and treated with vehicle (VEH), 25 nM dihydrotestosterone (DHT) or 5 μM PF-573228 (PF). All samples were probed with PXN and AR antibodies. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Scale bar: 10 μm. AR: androgen receptor, PXN: paxillin, DNMT: <t>DNA</t> <t>methyltransferase</t> 1, GC: granulosa cells, VEH: vehicle, PF: PF-573228, FAK: focal adhesion kinase, CRISPR: clustered regularly interspaced short palindromic repeats, PXN KO: paxillin knockout, NT CTRL: non-targeting control. Uncropped blots are shown in .
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    Androgen receptor and paxillin interaction requires intact granulosa cell focal adhesions. ( A ) Proximity ligation assay (PLA) in primary mouse GCs treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and probed with antibodies against AR and PXN or AR and <t>DNMT</t> as negative control. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Right: red fluorescence was quantified in five randomly selected cells in each sample using Image J and analyzed with Mann–Whitney tests. Magnification: ×40. ** P < 0.01. ( B ) Representative western blot and band densitometry of primary mouse GCs (n = 3–6 mice per group) treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and analyzed by Mann–Whitney tests. * P < 0.05. ( C ) PLA assay in KGN cells edited with CRISPR to delete paxillin (PXN KO) or non-targeting control (NT CTRL) and treated with vehicle (VEH), 25 nM dihydrotestosterone (DHT) or 5 μM PF-573228 (PF). All samples were probed with PXN and AR antibodies. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Scale bar: 10 μm. AR: androgen receptor, PXN: paxillin, DNMT: <t>DNA</t> <t>methyltransferase</t> 1, GC: granulosa cells, VEH: vehicle, PF: PF-573228, FAK: focal adhesion kinase, CRISPR: clustered regularly interspaced short palindromic repeats, PXN KO: paxillin knockout, NT CTRL: non-targeting control. Uncropped blots are shown in .
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    dna methyltransferases (dnmt) 1 antibody  (ZenBio)
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    Androgen receptor and paxillin interaction requires intact granulosa cell focal adhesions. ( A ) Proximity ligation assay (PLA) in primary mouse GCs treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and probed with antibodies against AR and PXN or AR and <t>DNMT</t> as negative control. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Right: red fluorescence was quantified in five randomly selected cells in each sample using Image J and analyzed with Mann–Whitney tests. Magnification: ×40. ** P < 0.01. ( B ) Representative western blot and band densitometry of primary mouse GCs (n = 3–6 mice per group) treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and analyzed by Mann–Whitney tests. * P < 0.05. ( C ) PLA assay in KGN cells edited with CRISPR to delete paxillin (PXN KO) or non-targeting control (NT CTRL) and treated with vehicle (VEH), 25 nM dihydrotestosterone (DHT) or 5 μM PF-573228 (PF). All samples were probed with PXN and AR antibodies. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Scale bar: 10 μm. AR: androgen receptor, PXN: paxillin, DNMT: <t>DNA</t> <t>methyltransferase</t> 1, GC: granulosa cells, VEH: vehicle, PF: PF-573228, FAK: focal adhesion kinase, CRISPR: clustered regularly interspaced short palindromic repeats, PXN KO: paxillin knockout, NT CTRL: non-targeting control. Uncropped blots are shown in .
    Dna Methyltransferases (Dnmt) 1 Antibody, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti dnmt 1  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology anti dnmt 1
    Androgen receptor and paxillin interaction requires intact granulosa cell focal adhesions. ( A ) Proximity ligation assay (PLA) in primary mouse GCs treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and probed with antibodies against AR and PXN or AR and <t>DNMT</t> as negative control. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Right: red fluorescence was quantified in five randomly selected cells in each sample using Image J and analyzed with Mann–Whitney tests. Magnification: ×40. ** P < 0.01. ( B ) Representative western blot and band densitometry of primary mouse GCs (n = 3–6 mice per group) treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and analyzed by Mann–Whitney tests. * P < 0.05. ( C ) PLA assay in KGN cells edited with CRISPR to delete paxillin (PXN KO) or non-targeting control (NT CTRL) and treated with vehicle (VEH), 25 nM dihydrotestosterone (DHT) or 5 μM PF-573228 (PF). All samples were probed with PXN and AR antibodies. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Scale bar: 10 μm. AR: androgen receptor, PXN: paxillin, DNMT: <t>DNA</t> <t>methyltransferase</t> 1, GC: granulosa cells, VEH: vehicle, PF: PF-573228, FAK: focal adhesion kinase, CRISPR: clustered regularly interspaced short palindromic repeats, PXN KO: paxillin knockout, NT CTRL: non-targeting control. Uncropped blots are shown in .
    Anti Dnmt 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt-1+antibody/Dnmt1+Antibody/pm34003488-51-9-17
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    Image Search Results


    Androgen receptor and paxillin interaction requires intact granulosa cell focal adhesions. ( A ) Proximity ligation assay (PLA) in primary mouse GCs treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and probed with antibodies against AR and PXN or AR and DNMT as negative control. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Right: red fluorescence was quantified in five randomly selected cells in each sample using Image J and analyzed with Mann–Whitney tests. Magnification: ×40. ** P < 0.01. ( B ) Representative western blot and band densitometry of primary mouse GCs (n = 3–6 mice per group) treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and analyzed by Mann–Whitney tests. * P < 0.05. ( C ) PLA assay in KGN cells edited with CRISPR to delete paxillin (PXN KO) or non-targeting control (NT CTRL) and treated with vehicle (VEH), 25 nM dihydrotestosterone (DHT) or 5 μM PF-573228 (PF). All samples were probed with PXN and AR antibodies. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Scale bar: 10 μm. AR: androgen receptor, PXN: paxillin, DNMT: DNA methyltransferase 1, GC: granulosa cells, VEH: vehicle, PF: PF-573228, FAK: focal adhesion kinase, CRISPR: clustered regularly interspaced short palindromic repeats, PXN KO: paxillin knockout, NT CTRL: non-targeting control. Uncropped blots are shown in .

    Journal: Molecular Human Reproduction

    Article Title: Paxillin regulates androgen receptor expression associated with granulosa cell focal adhesions

    doi: 10.1093/molehr/gaae018

    Figure Lengend Snippet: Androgen receptor and paxillin interaction requires intact granulosa cell focal adhesions. ( A ) Proximity ligation assay (PLA) in primary mouse GCs treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and probed with antibodies against AR and PXN or AR and DNMT as negative control. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Right: red fluorescence was quantified in five randomly selected cells in each sample using Image J and analyzed with Mann–Whitney tests. Magnification: ×40. ** P < 0.01. ( B ) Representative western blot and band densitometry of primary mouse GCs (n = 3–6 mice per group) treated for 24 h with 5 μM PF-573228 (PF) or DMSO (VEH) and analyzed by Mann–Whitney tests. * P < 0.05. ( C ) PLA assay in KGN cells edited with CRISPR to delete paxillin (PXN KO) or non-targeting control (NT CTRL) and treated with vehicle (VEH), 25 nM dihydrotestosterone (DHT) or 5 μM PF-573228 (PF). All samples were probed with PXN and AR antibodies. Protein proximity signal produces red fluorescence, merged with DAPI indicating cell nuclei. Scale bar: 10 μm. AR: androgen receptor, PXN: paxillin, DNMT: DNA methyltransferase 1, GC: granulosa cells, VEH: vehicle, PF: PF-573228, FAK: focal adhesion kinase, CRISPR: clustered regularly interspaced short palindromic repeats, PXN KO: paxillin knockout, NT CTRL: non-targeting control. Uncropped blots are shown in .

    Article Snippet: For DNA methyltransferase (DNMT), the Novus Biologicals catalog #NB100-56519 antibody was used at 1:100 dilution (10 μg/ml, Centennial, CO, USA), and for cytoplasmic poly (A)-binding protein (PABP), the Abcam catalog #21060 antibody was used at 1:100 dilution (10 μg/ml).

    Techniques: Proximity Ligation Assay, Negative Control, Fluorescence, MANN-WHITNEY, Western Blot, CRISPR, Control, Knock-Out